| Autor: |
Chaĭka OV, Khil'ko SN, Grodnitskaia NA, Dreĭzin RS, Tikhonenko TI |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 1982 Jul-Aug; Vol. 27 (4), pp. 422-6. |
| Abstrakt: |
A total preparation of cytoplasmic RNA was isolated from late stage-infected African green monkey kidney cells using phenol extraction procedure. The poly (A)-containing mRNA fraction was selected on oligo(dT)-cellulose columns. The resulting mRNA preparations were heterogenous in size and contained about 20--60% of SA7-derived sequences. SA7 late mRNA was efficiently translated in rabbit reticulocyte cell-free system giving rise to a number of polypeptide products that were related by antigenicity to authentic SA7 virion proteins. The main translation product having a molecular weight of 114 kilodaltons was identified as intact SA7 hexon protein. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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