| Abstrakt: |
A sensitive, specific GC assay for imidazopyrazole in plasma was developed using nitrogen-specific detection. The samples are extracted with methylene chloride containing 7-bromo-imidazopyrazole as the internal standard and the extract derivatized with pentaflourobenzoyl chloride prior to isothermal chromatography on an OV-17 column. Peak-height ratio measurements produced linear standard curves over the concentration range of 0.045-40 microgram/ml. The practical limit of sensitivity was 50 ng/ml and typical between-run variability for replicate analysis of a control specimen produced a coefficient of variation of 5.1%. This method is applicable to the study of the pharmacokinetics of imidazopyrazole following therapeutic doses and was used to support such studies in parallel with Phase I clinical studies in children. |
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