| Autor: |
Vikhnovich EM, Zolotarskaia EE, Loparev VN, Dreĭzin RS |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 1982 May-Jun; Vol. 27 (3), pp. 365-9. |
| Abstrakt: |
The clone KB-230 of simian adenovirus SA7(C8) is described differing from the reference SA7(C8) strain and clones KB-2 and MB-1 by the presence of additional recognition sites when treated with different endonucleases. The KB-230 clone differs antigenically in the neutralization test from the MB-1 clone. Some biological and molecular-biological properties of the KB-230 clone were studied. All the simian cell cultures under study were highly sensitive to the cytopathic effect and replication of the KB-230 clone. The reproduction cycle of the KB-230 clone was 12 h, that of KB-2 clone 16 h. The maximum accumulation of virus in the cells was observed by 27-29 h for both clones. The KB-230 clone differed from the KB-2 clone by early development of the CPE (by 9 h of cultivation against 19 h for the latter). The oncogenic activity of the KB-230 clone was less marked than that of the MB-1 clone. The methods of heteroduplex and polypeptide analysis established the difference of the KB-230 clone from the reference SA7(C8) strain and KB-2 and MB-1 clones. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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