| Autor: |
Oud PS, Zahniser DJ, Harbers-Hendriks R, van Boekel MC, Raaijmakers MC, Vooijs PG |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical and quantitative cytology [Anal Quant Cytol] 1981 Mar; Vol. 3 (1), pp. 73-9. |
| Abstrakt: |
The preparation procedure for the BioPEPR automated image analysis system is described. Cervical cells are collected in a preservative solution and disaggregated by an automated syringing apparatus, giving about 50% single cells. After centrifugation, the preservative solution is discarded, 2% polyethyleneglycol in 50% ethanol (carbowax) is added, and the cell solution is automatically spread onto a glass slide, resulting in a cell density of about 50 cells/sq mm in the central area of the smear. Increasing cell density is shown to lead to a gradual linear decrease in single cells. All preparation steps have been critically evaluated, and the possibility of implementing this preparation procedure in a fully automated system is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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