| Autor: |
Surguchov AP, Fominykch ES, Berestetskaya YV, Smirnov VN, Inge-Vechtomov SG |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular & general genetics : MGG [Mol Gen Genet] 1980; Vol. 177 (4), pp. 675-80. |
| DOI: |
10.1007/BF00272679 |
| Abstrakt: |
The shift of recessive suppressor mutant of yeast Saccharomyces cerevisiae from permissive to restrictive conditions is accompanied by polysome decay and accumulation of 80 S ribosomes (Smirnov et al., 1976). In this paper some properties of 80 S ribosomes are studied. It is demonstrated that polysome decay under non-permissive conditions is not the consequence of the impairment of RNA synthesis. More than 70% of 80 S ribosomes accumulated under non-permissive conditions contain bound peptidyl-tRNAs localized in P-ribosomal site. tRNA moiety of bound peptidyl-tRNA is able to accept all 20 natural amino acids after chemical deacylation. Therefore it is not a specific isoacceptor species but rather total tRNA that is bound to ribosomes. The polypeptide residues of these peptidyl-tRNAs are heterogeneous in size. Their molecular weights are comparable with the molecular weights of the completed polypeptides. Some of the 80 S ribosomes accumulated under non-permissive conditions contain poly-A+ RNA. In conclusion, possible mechanism of the impairment of translation under non-permissive conditions in recessive suppressor strain is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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