| Autor: |
Curfman GD, O'Hara DS, Hopkins BE, Smith TW |
| Jazyk: |
angličtina |
| Zdroj: |
Circulation research [Circ Res] 1980 Apr; Vol. 46 (4), pp. 581-9. |
| DOI: |
10.1161/01.res.46.4.581 |
| Abstrakt: |
To study the effects of leucine, glucose, and insulin on myocardial protein degradation in fed and fasted nutritional states, we developed and validated a sensitive method for measuring rates of total protein degradation in rat isolated left atrial preparations. Fasting resulted in a progressive decrease in myocardial protein breakdown to 71% of control over a 24-hour period, with no further reduction in degradation rate between 24 and 72 hours of fasting. Insulin (100 mU/ml) suppressed atrial protein degradation by 38% in fed animals (P less than 0.001) and by 51% in fasted animals (P less than 0.001). Glucose alone had no effect on protein degradation in either nutritional state. At 5 times normal plasma levels, leucine suppressed protein breakdown by 21% in fed and by 15% in fasted animals. The decrease in degradation induced by fasting and the absence of an effect of glucose are in contrast to the behavior reported for skeletal muscle. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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