Methodological issues in the measurement of urinary MHPG.
| Autor: | Muscettola G, Potter WZ, Gordon EK, Goodwin FK |
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| Jazyk: | angličtina |
| Zdroj: | Psychiatry research [Psychiatry Res] 1981 Jun; Vol. 4 (3), pp. 267-76. |
| DOI: | 10.1016/0165-1781(81)90028-7 |
| Abstrakt: | Unlabelled: Reported mean values for urinary 3-methoxy-4-hydroxyphenylglycol (MHPG) for both depressed patients and controls vary substantially between laboratories. The present study describes methodological sources of variance affecting urinary MHPG values. Although intra-assay variance is small (coefficient of variation (CV) = 4.6%), the interassay variance is high (CV = 14.3%) and suggests caution in the interpretation of small group differences. The gas chromatographic (GC) method in this laboratory produces values highly correlated (r = 0.98) with those obtained by a reference gas chromatographic-mass spectrometric (GC-MS) method. The absolute values obtained by GC are 20% higher than those by GC-MS--a problem that may be shared to a varying extent by all GC assays. In Conclusion: methodological differences do contribute to some of the variation in reported values of urinary MHPG. |
| Databáze: | MEDLINE |
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