| Autor: |
Griffiss JM, Bertram MA, Broud DD |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1978 Aug 21; Vol. 156 (1), pp. 121-30. |
| DOI: |
10.1016/s0021-9673(00)83132-5 |
| Abstrakt: |
A continuous, in-line chromatographic process for the separation of immunoglobulins M, A and G from small volumes of human sera is described. The process involves the sequential and uninterrupted application of molecular sieve, ion-exchange, immunoadsorbent-affinity and de-saltin-concentrating chromatographic methodologies. From 1-3 ml of starting serum it yields 45-55%, by weight, of the three classes of immunoglobulins in 36 h. Each immunoglobulin fraction is free of contamination by immunoglobulins of the other two classes and retains full biological activity as measured by a sensitive immune lysis assay. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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