| Autor: |
Anderson DP, Merchant B, Dixon OW, Schott CF, Lizzio EF |
| Jazyk: |
angličtina |
| Zdroj: |
Developmental and comparative immunology [Dev Comp Immunol] 1983 Spring; Vol. 7 (2), pp. 261-8. |
| DOI: |
10.1016/0145-305x(83)90007-1 |
| Abstrakt: |
Rainbow trout (Salmo gairdneri) were immunized by flush exposure or intraperitoneal injection with single doses of the following dinitrophenyl (DNP) conjugates: DNP-keyhole limpet hemocyanin (KLH), DNP-Ficoll, DNP-O-antigen (from the fish pathogen, Yersinia ruckeri), DNP-sheep red blood cells, or DNP-duck red blood cells. The immune response was demonstrated by the passive hemolytic plaque assay to show splenic antibody-producing cells (APC) 14 days after antigen administration. High numbers of splenic APC specific for the hapten were found when the conjugates DNP-KLH, DNP-Ficoll, and DNP-O-antigen were given by injection and when DNP-O-antigen was given by flush exposure. The heterologous red blood cells were relatively nonimmunogenic. When the immune response to the hapten and carrier could be measured--e.g., after immunization with the DNP-O-antigen--the numbers of APC were consistently higher for the hapten. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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