| Autor: |
Newton CR, Greene AR, Heathcliffe GR, Atkinson TC, Holland D, Markham AF, Edge MD |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 1983 Feb 15; Vol. 129 (1), pp. 22-30. |
| DOI: |
10.1016/0003-2697(83)90046-5 |
| Abstrakt: |
The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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