| Autor: |
Britton DW, Lawrie JS, Kemp GD |
| Jazyk: |
angličtina |
| Zdroj: |
Thrombosis research [Thromb Res] 1982 Jul 15; Vol. 27 (2), pp. 167-73. |
| DOI: |
10.1016/0049-3848(82)90196-7 |
| Abstrakt: |
Fibrinogen fragment D prepared in the presence of calcium ions (fragment D[Ca++]) shows qualitatively similar cross-linking patterns with dimethyl suberimidate, dimethyl adipimidate and tetranitromethane. Fragment D prepared in the presence of EDTA (fragment D[EDTA]) gives a consistently different pattern with these reagents. In the case of fragment D[EDTA] there is much more intermolecular cross-linking suggesting that the loss of the C-terminus of the gamma-chain remnant results in fragment D adopting a more open conformation. Neither the addition of 2M urea nor EDTA to fragment D[Ca++] alters its cross-linking pattern suggesting that the proposed conformational change follows cleavage of a plasmin susceptible bond which is normally protected by the presence of calcium ions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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