Detection of Neisseria gonorrhoeae by ultraviolet illumination of NADH generated by an immobilized antibody-gonococcal enzyme complex.

Autor: McDonald HC, Simon PA, Takeguchi MM, Odstrchel G, Weetall HH, Felman YM
Jazyk: angličtina
Zdroj: Immunology letters [Immunol Lett] 1981 Dec; Vol. 3 (6), pp. 377-80.
DOI: 10.1016/0165-2478(81)90070-5
Abstrakt: Frozen, cervical swabs were placed in a lysis buffer containing an immobilized antibody to the gonococcal enzyme, 1,2-propanediol oxidoreductase. The immobilized antibody--enzyme complex that formed was active after the addition of substrate (1,2-propanediol and NAD) and this activity could be detected by visual inspection of NADH fluorescence under ultraviolet illumination.
Databáze: MEDLINE