| Autor: |
Eckels KH, Harrison VR, Summers PL, Russell PK |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 1980 Jan; Vol. 27 (1), pp. 175-80. |
| DOI: |
10.1128/iai.27.1.175-180.1980 |
| Abstrakt: |
The S-1 clone of dengue type 2 virus was used for the preparation of a live-attenuated vaccine after passage in DBS-FRhL-2 cell culture. The vaccine virus had a relatively higher replicative capacity at superoptimal temperatures than its precursor virus, S-1, passaged in primary green monkey kidney cells (S-1 PGMK). There was also a tendency for the S-1 vaccine virus to exhibit leakiness at increased temperatures. Another in vitro marker, replication in monkey peripheral blood leukocytes, indicated less host restriction for the S-1 vaccine in comparative assays with S-1 PGMK virus. Mouse virulence appeared to remain stable on passage in DBS-FRhL-2 cells, whereas monkey immunogenicity decreased. Cautious trials of the dengue type 2 S-1 vaccine in humans are indicated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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