| Autor: |
Seeley DH, Mester J, Baulieu EE, Wolfson AJ |
| Jazyk: |
angličtina |
| Zdroj: |
Endocrinology [Endocrinology] 1984 May; Vol. 114 (5), pp. 1740-4. |
| DOI: |
10.1210/endo-114-5-1740 |
| Abstrakt: |
The estrogen receptor was extracted in high yield from nuclei of laying hen oviduct with 10 mM pyridoxal-5'-phosphate (PLP). The receptor extracted under these conditions, unlike that extracted with 0.4 M KCl, displayed no tendency to aggregate on sucrose gradients in low salt. The receptor was eluted as a single peak from DEAE-Sephacel at an ionic strength of 0.13 M KCl. The receptor after DEAE chromatography had approximately half the molecular weight of that in the nuclear extract. A larger form could be reconstituted by the addition of whole nuclear extract to the DEAE eluate. These data support the notion that the nuclear estrogen receptor is a dimer composed of similar subunits. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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