| Autor: |
Fay DD, Madden JF, Costa JL |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1984 Feb 29; Vol. 119 (1), pp. 116-23. |
| DOI: |
10.1016/0006-291x(84)91626-7 |
| Abstrakt: |
A technique has been developed for quantitating the absolute number of dense bodies present in solution following isolation from human platelets. The amount of [3H]-5HT accumulated per dense body was measured following either sonication alone or sonication plus isolation utilizing a Metrizamide density gradient; the dense bodies in each case were washed and resuspended in sodium or potassium-rich buffer. Uptake per dense body following removal from the cell was less than 10% of the amount of uptake per dense body by intact platelets. It thus seems possible that residence of dense bodies inside intact platelets is required for 5HT transport into dense bodies to proceed at a maximal rate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
