Leishmanial excreted factors (EFs): purification by affinity chromatography.

Autor: Zehavi U, Abrahams JC, Granoth R, Greenblatt CL, Slutzky GM, El-On J
Jazyk: angličtina
Zdroj: Zeitschrift fur Parasitenkunde (Berlin, Germany) [Z Parasitenkd] 1983; Vol. 69 (6), pp. 695-701.
DOI: 10.1007/BF00927419
Abstrakt: Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.
Databáze: MEDLINE