| Autor: |
Bauza MT, Smith RV, Knutson DE, Witter FR |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1984 Sep 14; Vol. 310 (1), pp. 61-9. |
| DOI: |
10.1016/0378-4347(84)80068-7 |
| Abstrakt: |
A method has been developed for determination of the xanthine drug, pentoxifylline, and three of its metabolites (a secondary alcohol and two carboxylic acids) in human milk. The method requires pre-extraction with hexane to remove lipids followed by extraction with dichloromethane or dichloromethane-isopropanol (4:1). Absolute extraction recoveries were between 76-90%. Pentoxifylline and its alcohol metabolite (as the trifluoroacetate) and the carboxylic acid metabolites (as ethyl esters) were measured in separate gas chromatographic steps using a nitrogen detector. Determinations of pentoxifylline and its three metabolites were 96-99% accurate and standard deviations of 5-10% were observed for samples at or above the lower practical sensitivity limit (10 ng/ml) for the assay. Pentoxifylline and its metabolites were stable in breast milk for three weeks when stored at -15 degrees C. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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