| Autor: |
Sinha AK, Linscombe VA, Gollapudi BB, McClintock ML, Flake RE, Bodner KM |
| Jazyk: |
angličtina |
| Zdroj: |
Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie [Can J Genet Cytol] 1984 Oct; Vol. 26 (5), pp. 528-31. |
| DOI: |
10.1139/g84-084 |
| Abstrakt: |
Spontaneously occurring cytogenetic aberrations were compared in 48- and 72-h human lymphocyte cultures from a group of 20 normal male volunteers. Mitotic indices for both cultures times were also evaluated. BUdR (5-bromo, 2-deoxyuridine) labelled metaphases from three men were utilized to determine the cell cycle kinetics in the medium used. There was no statistically significant difference between 48- and 72-h cultures among the majority of aberration categories considered. Possibly, the lymphocyte populations at the two different culture times maintained a consistent yield level. Mitotic indices in 72-h cultures were invariably higher than those in 48-h cultures. An overwhelming number of first division metaphases were observed at 48 h of incubation; however, approximately 22% of the lymphocytes did not enter into mitosis until 72 h in culture. A number of difficulties with regard to utilizing BUdR-labelled first division metaphases for cytogenetic analyses are discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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