| Autor: |
McGovern EP, Swynnerton NF, Steele PD, Mangold DJ |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of radiation oncology, biology, physics [Int J Radiat Oncol Biol Phys] 1984 Sep; Vol. 10 (9), pp. 1517-20. |
| DOI: |
10.1016/0360-3016(84)90493-0 |
| Abstrakt: |
A high pressure liquid chromatography (HPLC) plasma assay for WR-1065 is described which is both precise (coefficient of variation less than 5%) and accurate (% average deviation less than or equal to 6.1) throughout the concentration range from 1 to 500 micrograms/mL of plasma. The analyte is separated by HPLC and detected with a thiol specific electrochemical transducer cell. The detector response is linear over the ranges 1 to 10 micrograms/mL (R2 = 0.995), 10 to 100 micrograms/mL (R2 = 0.995), and 100 to 500 micrograms/mL (R2 = 0.974). The absolute retention times for WR-1065 and WR-1729 are 9 and 12 minutes, respectively. The assay uses 100 microL of plasma and requires a total chromatography cycle time of 40 minutes. The method has been found suitable for the determination of WR-1065 in plasma from a beagle dog after i.v. administration of S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
