CFU-GM colony-enhancing activity in sera of dogs under acute and chronic gamma-irradiation regimens.
| Autor: | Kaspar LV, Seed TM |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Acta haematologica [Acta Haematol] 1984; Vol. 71 (3), pp. 189-97. |
| DOI: | 10.1159/000206583 |
| Abstrakt: | Dogs were chronically irradiated (10 R/day; 0-400 R) or acutely irradiated (20 R/min; 0-400 R) with 60Co gamma-rays. Sera were collected and assayed for colony-enhancing activity by a double-layer agar cloning technique. When test sera alone were incorporated in the feeder layers, no colony-forming units-granulocyte/macrophage formation occurred; the addition of peripheral blood leukocytes to such test sera-containing feeder layers resulted in formation of colonies, the number of which was directly related to the cumulative radiation dose. Serum-enhancing factor activity indirectly increased colony formation by stimulating feeder layer leukocytes to generate increased levels of colony-stimulating activity. Levels of serum-enhancing activity were higher at similar dose levels following acute gamma irradiation then following chronic irradiation. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|