Plasmid-mediated complementation of a delta-aminolevulinic-acid-requiring Saccharomyces cerevisiae mutant.

Autor: Bard M, Ingolia TD
Jazyk: angličtina
Zdroj: Gene [Gene] 1984 May; Vol. 28 (2), pp. 195-9.
DOI: 10.1016/0378-1119(84)90256-7
Abstrakt: Recombinant plasmids able to complement the Saccharomyces cerevisiae ole3 mutation were isolated. The nucleotide sequence responsible for complementation was localized to a 3.5-kb region. The level of delta-aminolevulinate (ALV) synthase activity in wild-type cells was six-fold lower than in plasmid-transformed ole3 mutant cells. Certain clones secreted a compound that supported growth of a lawn of adjacent ole3 mutant cells.
Databáze: MEDLINE