Confirmation of EMIT cannabinoid assay results by bonded phase adsorption with thin layer chromatography.

Autor: Willson NJ, Kogan MJ, Pierson DJ, Newman E
Jazyk: angličtina
Zdroj: Journal of toxicology. Clinical toxicology [J Toxicol Clin Toxicol] 1983 Jul; Vol. 20 (5), pp. 465-73.
DOI: 10.3109/15563658308990612
Abstrakt: Clinical urine specimens were screened for the presence of cannabinoids using the EMIT Cannabinoid Assay. Aliquots of these samples were also analyzed for 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THCA), the major cannabis metabolite in urine, by a technique which combines bonded phase adsorption (BPA) and thin layer chromatography (TLC). A 100% agreement between EMIT and BPA-TLC results was observed when at least 20 mL of urine was assayed by BPA-TLC. Bonded phase adsorption coupled with thin layer chromatography appears to be a suitable technique for the confirmation of positive EMIT Cannabinoid Assay results.
Databáze: MEDLINE