| Autor: |
Salmon M, Bacon PA, Johnson GD, Walker L, Walton KW |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1984 Feb 10; Vol. 66 (2), pp. 327-30. |
| DOI: |
10.1016/0022-1759(84)90345-4 |
| Abstrakt: |
Cytocentrifuged cell preparations have been evaluated as substrates for the demonstration by immunofluorescence of T cell surface antigens. Results obtained by a 2-stage method with fluorescent conjugate specific for the isotype of the first stage monoclonal antibody, and by a 3-stage procedure employing an intermediate antiserum to mouse IgG as amplifying agent, showed good correlation with those obtained by a standard technique performed on cells in suspension. These methods have the advantages of requiring fewer cells and less antibody and the cytocentrifuge preparations may be stored for batch assay. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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