| Autor: |
Wovcha MG, Steuerwald DL, Brooks KE |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 1983 Apr; Vol. 45 (4), pp. 1402-4. |
| DOI: |
10.1128/aem.45.4.1402-1404.1983 |
| Abstrakt: |
A recombinant plasmid, designated pUC1002, was constructed by ligation of a HindIII restriction endonuclease fragment of Escherichia coli chromosomal DNA to vector plasmid pMB9. Strains carrying this plasmid were selected by transformation of an E. coli strain bearing the xyl-7 mutation to a xylose-positive (Xyl+) phenotype. Strains containing pUC1002 produced coordinately elevated levels of D-xylose isomerase and D-xylulose kinase. Under appropriate conditions, the isomerase also efficiently catalyzed the conversion of D-glucose to D-fructose. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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