| Autor: |
Zabucchi G, Rottini GD, Soranzo MR, Tedesco F, Patriarca P |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1983 Feb 25; Vol. 57 (1-3), pp. 253-64. |
| DOI: |
10.1016/0022-1759(83)90085-6 |
| Abstrakt: |
A simple and rapid spectrophotometric assay for the kinetic evaluation of serum-induced damage to E. coli is described, based on changes in the optical density (OD) of a bacterial suspension. Exposure of antibody-coated E. coli to human absorbed serum results in a diphasic response, namely an increase in OD, which reaches a maximum at about 17 min and is followed by a progressive decrease in OD until a minimum value is reached after 45 min. The increase and the decrease in OD are related to bacterial death and bacterial lysis, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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