| Autor: |
Valyakina TI, Vershinina NV, Gilyarevskii SD, Nesmeyanov VA |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular immunology [Mol Immunol] 1984 Sep; Vol. 21 (9), pp. 811-4. |
| DOI: |
10.1016/0161-5890(84)90168-8 |
| Abstrakt: |
The production of interleukin 2 by rat splenocytes, stimulated with Con A in serum-free medium, was studied. Under optimal conditions the stimulation in serum-containing and serum-free media resulted in identical interleukin 2 titers. The addition of phorbol myristate acetate prior or simultaneously with Con A did not augment interleukin 2 production. In contrast, preincubation of lymphocytes in serum-free medium for 24-36 hrs brought about a 8-10-fold increase of interleukin 2 titer. The interleukin 2 titer 4 hrs after the induction of preincubated lymphocytes with Con A was about 20% of maximal activity. The possible mechanism of early interleukin 2 production is under discussion. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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