[Effect of cyclic nucleotides on the morphogenetic and ultrastructural characteristics of the primitive knot in the chick embryo in organotypic cultures].

Autor: Neuman TE, Laasberg TA, Kiarner IuK
Jazyk: ruština
Zdroj: Ontogenez [Ontogenez] 1984 Jan-Feb; Vol. 15 (1), pp. 88-91.
Abstrakt: The isolated Hensen's nodes of chick embryos (stage 4 after Hamburger and Hamilton) were cultivated in organotypic cultures after the treatment with 0.5 mM solutions of cAMP and cGMP. Both the control and treated explants were characterized by a wide variability of differentiation of various tissue rudiments. cGMP inhibited reliably the differentiation of notochord. At the ultrastructural level the control and cAMP-treated cultures were characterized by an increased autophagia whereas the treatment with cGMP inhibited the yolk utilization and resulted in the appearance of vast regions of partial necrosis.
Databáze: MEDLINE