Growth and purification of SV40 virus for biochemical studies.

Autor: Rosenberg BH, Deutsch JF, Ungers GE
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 1981 Oct; Vol. 3 (3), pp. 167-76.
DOI: 10.1016/0166-0934(81)90051-3
Abstrakt: A detailed growth and purification scheme suitable for producing relatively large quantities of fully active, pure SV40 is presented together with data on recovery and purity at each step of the procedure. The scheme was designed to prevent the initial binding of virus to cell components as well as contamination of the extracted virus by cellular DNA.
Databáze: MEDLINE