Introduction of active enzymes into intact Escherichia coli cells by means of liposomes. Phenotypic suppression of uvr A and pol A mutants.

Autor: Bresler SE, Noskin LA, Kaboev OK, Stepanova IM, Davidenkova EF, Rosenberg OA, Schwartz EI, Beketova AG, Akhmedov AT
Jazyk: angličtina
Zdroj: Molecular & general genetics : MGG [Mol Gen Genet] 1981; Vol. 181 (4), pp. 532-4.
DOI: 10.1007/BF00428748
Abstrakt: Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons).
Databáze: MEDLINE