| Autor: |
Kapikian AZ, Cline WL, Greenberg HB, Wyatt RG, Kalica AR, Banks CE, James HD Jr, Flores J, Chanock RM |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 1981 Aug; Vol. 33 (2), pp. 415-25. |
| DOI: |
10.1128/iai.33.2.415-425.1981 |
| Abstrakt: |
An immune adherence hemagglutination assay (IAHA) and a modified enzyme-linked immunosorbent assay for antigenic characterization of human rotaviruses were developed. The designations of type 1 and type 2 were identical to those established previously by specific complement fixation, enzyme-linked immunosorbent assay, and immune electron microscopy. By IAHA (and modified enzyme-linked immunosorbent assay) certain animal rotaviruses were found to be closely related to human rotavirus type 1. The pattern of IAHA reactivity and the cell culture neutralization serotype were found to be distinct properties. The separation of neutralization and IAHA reactivity was apparent when animal rotaviruses which were distinguishable from each other by neutralization assays were found to share IAHA specificity. Further evidence for the dissociation of the neutralization and IAHA specificities was found in studies of human and bovine rotaviruses which underwent genetic reassortment during coinfection. Thus, it appeared that the IAHA and neutralization antigens were coded for by different genes. In view of these findings, we suggest that the term serotype be reversed to identify the antigen that reacts with neutralizing antibodies as is customary for other viruses and that the term subgroup (instead of serotype) be used for the specificity detected by specific complement fixation, enzyme-linked immunosorbent assay, and now IAHA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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