Autor: |
Edvabnaya LS, Stanislavskii ES, Lany B, Schiller B, Bichowicz I |
Jazyk: |
angličtina |
Zdroj: |
Journal of hygiene, epidemiology, microbiology, and immunology [J Hyg Epidemiol Microbiol Immunol] 1981; Vol. 25 (2), pp. 163-8. |
Abstrakt: |
O-sera were obtained by immunizing rabbits with artificial antigens: polysaccharide extracted from the lipopolysaccharide of P. aeruginosa (strain No. 868; 03a, 3d, 3e), or the high-molecular-weight or low-molecular-weight fraction of this polysaccharide, complexed with a natural protein (human IgG or rabbit globulin). The antisera to these antigenic complexes were highly O-specific. Antisera to the complexes of polysaccharide-protein and high-molecular-weight fraction-protein were more active in the passive haemagglutination reaction, slide agglutination test and immunodiffusion test in agar gel than was antiserum to the low-molecular-weight fraction-protein complex. The artificial antigens prepared and employed in the study are apt to be used for the preparation of monospecific immune sera. |
Databáze: |
MEDLINE |
Externí odkaz: |
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