| Autor: |
Phillips LA, Pang RH, Park JJ, Hollis VW Jr, Famuyiwa F |
| Jazyk: |
angličtina |
| Zdroj: |
Preparative biochemistry [Prep Biochem] 1980; Vol. 10 (1), pp. 11-26. |
| DOI: |
10.1080/00327488008061715 |
| Abstrakt: |
These studies were done to determine four basic intrinsic properties of poly(U)-agarose affinity columns. Specificity of binding studies demonstrated that binding to these columns is highly specific with greater than 90% complementary binding and less than or equal to 3% noncomplementary binding. Sensitivity of binding studies indicated that a minimum sequence of 10 adenylates is required for detectable complementary binding. Selectivity of binding studies revealed that nonsequential adenylates in native RNAs and randomly distributed adenylates in synthetic poly(A)-poly(C) co-polymers did not bind to poly(U)-agarose affinity columns. Whereas, affinity of binding studies demonstrated that A=U complementary base pairing is independent of chain-lengths of greater than or equal to 25 adenylates and dependent of chain-lengths of less than 25 adenylates. Thus the data demonstrates that poly(U)-agarose affinity chromatography is scientifically sound and expedient for the detection and isolation of poly(A)-containing cellular and viral RNAs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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