Non-immunological precipitation by the neutral detergent triton X-100 in agar gel diffusion.

Autor: Mansheim BJ, Stenstrom ML
Jazyk: angličtina
Zdroj: Applied and environmental microbiology [Appl Environ Microbiol] 1980 Dec; Vol. 40 (6), pp. 1139-41.
DOI: 10.1128/aem.40.6.1139-1141.1980
Abstrakt: Triton X-100 can be used to clarify vague immunoprecipitin lines from bacterial antigens; however, non-immunological precipitation can lead to mistaken interpretation of immunodiffusion results. If Triton X-100 is added directly to the gel during preparation rather than to the antigen well, this detergent artifact can be eliminated.
Databáze: MEDLINE