Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.

Autor: Wimber DE, Steffensen DM
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 1970 Nov 06; Vol. 170 (3958), pp. 639-41.
DOI: 10.1126/science.170.3958.639
Abstrakt: [(3)H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [(3)H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [(3)H]RNA and DNA was confined to the 56E-F segment.
Databáze: MEDLINE