Measurement of testosterone with a high-performance liquid chromatograph equipped with a flow-through ultraviolet spectrophotometer.

Autor: Cochran RC, Darney KJ Jr, Ewing LL
Jazyk: angličtina
Zdroj: Journal of chromatography [J Chromatogr] 1979 May 21; Vol. 173 (2), pp. 349-55.
DOI: 10.1016/s0021-9673(00)92303-3
Abstrakt: A technique is described for measuring nanogram amounts of testosterone using high-performance liquid chromatography with detection by a flow-through spectrophotometer. The addition of a non-radioisotopic internal standard (4-androsten-11 beta-ol-3,17-dione) to the biological specimen automatically corrects for testosterone losses due to extraction and non-quantitative sample injection into the high-performance liquid chromatograph. This new method, which can be performed by inexperienced personnel, is shown to be rapid, precise, accurate and specific for testosterone.
Databáze: MEDLINE