| Autor: |
Waner JL, Budnick JE |
| Jazyk: |
angličtina |
| Zdroj: |
Applied microbiology [Appl Microbiol] 1973 Jan; Vol. 25 (1), pp. 37-9. |
| DOI: |
10.1128/am.25.1.37-39.1973 |
| Abstrakt: |
A fluorescing cell assay (FCA) technique utilizing the indirect fluorescent-antibody method to measure human cytomegalovirus (CMV)-infected cells has been applied to the rapid determination of CMV-neutralizing antibody. Human sera with complement fixation titers to CMV of 1/32 or greater and fluorescein-conjugated rabbit anti-human globulin are the primary and secondary reagents in the fluorescent-antibody test. FCA measured in 3 days the same number of infectious units measured by plaque assay in 2 weeks. FCA and plaque assay yielded identical neutralizing antibody titers to CMV in 20 human sera. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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