A modified procedure for the preparation of UDP-beta-L-(U-14C)rhamnose.

Autor: Kamsteeg J, van Brederode J, Kuipers-Tierie E, van Nigtevecht G
Jazyk: angličtina
Zdroj: Preparative biochemistry [Prep Biochem] 1979; Vol. 9 (4), pp. 457-64.
DOI: 10.1080/00327487908061706
Abstrakt: This paper describes the synthesis of UDP-L-(U-14C)rhamnose from UDP-D-(U-14C)glucose and NADPH using an enzyme preparation of Nicotiana tabacum var. Xanthi. A procedure to separate UDP-l-rhamnose from the other compounds in the reaction mixture is described. Optimal separation was achieved in ethanol 95%-1 M ammonium acetate (pH 3.8) (7:3, v/v) at 30 degrees C.
Databáze: MEDLINE