Autor: |
Kamsteeg J, van Brederode J, Kuipers-Tierie E, van Nigtevecht G |
Jazyk: |
angličtina |
Zdroj: |
Preparative biochemistry [Prep Biochem] 1979; Vol. 9 (4), pp. 457-64. |
DOI: |
10.1080/00327487908061706 |
Abstrakt: |
This paper describes the synthesis of UDP-L-(U-14C)rhamnose from UDP-D-(U-14C)glucose and NADPH using an enzyme preparation of Nicotiana tabacum var. Xanthi. A procedure to separate UDP-l-rhamnose from the other compounds in the reaction mixture is described. Optimal separation was achieved in ethanol 95%-1 M ammonium acetate (pH 3.8) (7:3, v/v) at 30 degrees C. |
Databáze: |
MEDLINE |
Externí odkaz: |
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