Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.

Autor: Syrewicz JJ, Naso RB, Wang CS, Arlinghaus RB
Jazyk: angličtina
Zdroj: Applied microbiology [Appl Microbiol] 1972 Sep; Vol. 24 (3), pp. 488-94.
DOI: 10.1128/am.24.3.488-494.1972
Abstrakt: Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.
Databáze: MEDLINE