| Autor: |
Strongin AIa, Lukin AA, Izotova LS, Abramov ZT, Ermakova LM |
| Jazyk: |
ruština |
| Zdroj: |
Mikrobiologiia [Mikrobiologiia] 1977 May-Jun; Vol. 46 (3), pp. 539-46. |
| Abstrakt: |
Molecular forms of two exoenzymes, subtilisin and alpha-amylase, produced by mutants of Bacillus subtilis were studied. The degree of the post-translational modification of subtilisin was less pronounced for P and M mutants than for R mutants. Some of the P and M mutants produced subtilisin having higher molecular weight and hydrophobicity as compared to the R mutants. This form of subtilisin may be the primary product of translation of the structural gene and therefore a precursor of subtilisin. Its appearance outside the cell may be due to the alteration in the cell surface structures in the P and M mutants and abnormal post-translational modification. The P and M mutants produced also differing exocellular proteins as compared to the R mutants, e.g. alpha-amylase forms with the higher isoelectric points. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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