Mitochondrial calcium and cellular electrolytes in brain cortex frozen in situ: electron probe analysis.

Autor: Somlyo AP, Urbanics R, Vadasz G, Kovach AG, Somlyo AV
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1985 Nov 15; Vol. 132 (3), pp. 1071-8.
DOI: 10.1016/0006-291x(85)91916-3
Abstrakt: Mitochondrial calcium in rat brain cortex frozen in situ and measured with electron probe X-ray microanalysis was 1.5 mmol Ca/kg dry weight. Cellular, extramitochondrial Ca was 6.4mmol/kg dry weight and cytoplasmic Na+ was 73mM. These results do not support the role of mitochondria regulating cytoplasmic Ca2+, but are compatible with the regulation of mitochondrial enzymes by matrix free Ca2+.
Databáze: MEDLINE