| Autor: |
Arendt RM, Stangl E, Zähringer J, Liebisch DC, Herz A |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1985 Sep 09; Vol. 189 (1), pp. 57-61. |
| DOI: |
10.1016/0014-5793(85)80841-3 |
| Abstrakt: |
This paper describes a highly specific and sensitive radioimmunoassay for alpha-human atrial natriuretic factor (alpha-hANF), the C-terminal 28-amino-acid residue portion of human prepro-ANF in human plasma. A novel extraction and prepurification procedure allowed for detection of levels of immunoreactive-alpha-hANF as low as 0.5 fmol/ml. In normotensive subjects, levels in the range 1-23 fmol/ml (mean = 8.9 fmol/ml) were found. Combined gel permeation and HPLC analysis demonstrated that this ir-alpha-hANF was comprised virtually exclusively of authentic 28-residue alpha-hANF. No evidence for occurrence of larger precursor forms in human plasma was acquired. A heterogenous group of hypertensive patients displayed considerably higher levels (mean = 62.2 fmol/ml), of interest in view of the hypotensive properties of ANF. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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