Analysis of soybean sapogenins by high-performance liquid chromatography.

Autor: Ireland PA, Dziedzic SZ
Jazyk: angličtina
Zdroj: Journal of chromatography [J Chromatogr] 1985 May 29; Vol. 325 (1), pp. 275-81.
DOI: 10.1016/s0021-9673(00)96028-x
Abstrakt: A method for the analysis of soybean sapogenins is described. The method is based on the extraction of soybean saponins from a defatted sample. The triterpene glycosides are then hydrolysed with subsequent analysis of the liberated sapogenins by high-performance liquid chromatography using gradient elution and mass detection. By use of a sapogenin/carbohydrate ratio, an estimate of the total saponin content can be made.
Databáze: MEDLINE