[Study of the kinetics of thermoinactivation of lysyl-tRNA-synthetase from the liver of x-irradiated rats].
| Autor: | Verkhogliad IN, Vinogradova RP, Kucherenko NE |
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| Jazyk: | ruština |
| Zdroj: | Radiobiologiia [Radiobiologiia] 1985 Mar-Apr; Vol. 25 (2), pp. 185-90. |
| Abstrakt: | A study was made of the kinetics of thermoinactivation of lysyl-tRNA-synthetase isolated from rat liver at early times of radiation damage development after the effect of a minimum absolutely lethal X-radiation dose (0.21 C/kg). The thermostability of a dimer form of the enzyme was shown to be higher than that of a monomer. It was established that substrates had a stabilizing effect on the enzyme during thermoinactivation. On the basis of the data obtained from the studies in the kinetic properties of the enzyme and the thermoinactivation a conclusion is made that lysyl-tRNA-synthetase is stabilized during subunit aggregation. The thermostability of the enzyme was decreased by irradiation. |
| Databáze: | MEDLINE |
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