Microbial lysates as low-cost serum replacements in cellular agriculture media formulation.

Autor: Dolgin J; Department of Biomedical Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Chakravarty D; Department of Chemical & Biological Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Sullivan SF; Department of Chemical & Biological Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Cai Y; Department of Biology, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Lim T; Department of Biomedical Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Yamaguchi P; Department of Chemical & Biological Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Balkan JE; Department of Chemical & Biological Engineering, Tufts University Medford MA USA., Xu L; Department of Biomedical Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Olawoyin AD; Department of Biomedical Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Lee K; Department of Chemical & Biological Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Kaplan DL; Department of Biomedical Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA., Nair NU; Department of Chemical & Biological Engineering, Tufts University Medford MA USA; Tufts University Center for Cellular Agriculture (TUCCA), Tufts University Medford MA USA. Electronic address: nikhil.nair@tufts.edu.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2025 Feb; Vol. 201, pp. 115633. Date of Electronic Publication: 2025 Jan 03.
DOI: 10.1016/j.foodres.2024.115633
Abstrakt: Cultivated meat, the process of generating meat in vitro without sacrificing animals, is a promising alternative to the traditional practice of livestock agriculture. However, the success of this field depends on finding sustainable and economical replacements for animal-derived and expensive fetal bovine serum (FBS) that is typically used in cell culture processes. Here, we outline an effective screening process to vet the suitability of microbial lysates to support the growth of immortalized bovine satellite cells (iBSCs) and mackerel (Mack1) cells. We show that easily producible, low-cost whole-cell lysates from Vibrio natriegens can be used to create serum-free media for the long-term growth of iBSCs. The optimized medium, named "VN40" (basal B8 media containing Vibrio natriegens lysate proteins at 40 µg/mL), outperforms previously established serum-free media while maintaining cell phenotype and myogenicity. Overall, this study shows a novel approach to producing serum-free media for cultivated meat production using microbially-derived lysates.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nikhil U. Nair reports financial support was provided by Tufts University Center for Cellular Agriculture − Consortium. David L. Kaplan reports financial support was provided by Tufts University Center for Cellular Agriculture − Consortium. Kyongbum Lee reports financial support was provided by Tufts University Center for Cellular Agriculture − Consortium. David L. Kaplan reports financial support was provided by US Department of Agriculture. Nikhil U. Nair, David L. Kaplan, James Dolgin, Damayanti Chakravarty, Taehwan Lim, Sean F. Sullivan have patent pending to Tufts University. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2025 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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