Prevalence and Antibiotic Susceptibility of Pathogenic Enterobacteria Strains from Three Biotopes in the City of Ouagadougou (Burkina Faso).

Autor:	Dabiré SC; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso.; Département de Biochimie Microbiologie, Ecole Doctorale Sciences Et Technologies (EDST)/Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso., Somda MK; Département de Biochimie Microbiologie, Ecole Doctorale Sciences Et Technologies (EDST)/Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso., Nitièma LW; Institut de l'Environnement et de Recherches Agricoles (INERA), Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Kambiré D; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Kiemtoré S; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Soubeiga ST; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Zouré AA; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Yao KKT; Département de Biochimie Microbiologie, Ecole Doctorale Sciences Et Technologies (EDST)/Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso., Compaoré TR; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Ouedraogo HG; Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso., Dicko MH; Département de Biochimie Microbiologie, Ecole Doctorale Sciences Et Technologies (EDST)/Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
Jazyk:	angličtina
Zdroj:	Infection and drug resistance [Infect Drug Resist] 2024 Dec 30; Vol. 17, pp. 5909-5924. Date of Electronic Publication: 2024 Dec 30 (Print Publication: 2024).
DOI:	10.2147/IDR.S485200
Abstrakt:	Purpose: The emergence of antibiotic resistance in pathogenic Enterobacteriaceae is a public health problem in tropical countries such as Burkina Faso. Antibiotic resistance could be identified using a variety of approaches. This study aimed to estimate the prevalence of pathogenic enterobacteria strains from three sources, as well as their antibiotic resistance profile to biotope and climatic season. Material and Methods: The methodological approach consisted of identifying Enterobacteriaceae from human (urine, stool), animal (eggs, milk, fish), and environmental (soil, lettuce) samples, followed by assessing their antibiotic susceptibility. Samples were collected from February to December 2023. Bacterial species were isolated and phenotypically identified (morphologically, culturally, biochemically, and antigenically) using standard methods. The prevalence of bacterial susceptibility to ten antibiotics was determined using the agar disk diffusion method. The collected data were analyzed with IBM SPSS Statistics 25 software. Results: A total of 615 Enterobacteriaceae isolates were collected, including 300, 168, and 147 samples from human, animal, and environmental sources respectively. Phenotypic characteristics allowed to partially identify 43 species, among these 29.76% belonged to Escherichia coli , 24.72% to Enterobacter cloacae , 13. 82% to Klebsiella pneumoniae, 3.41% to Enterobacter sakazakii and 2.6% to Klebsiella oxytoca . Bacterial resistance rates were: aminopenicillins (54.8%), first-generation cephalosporins (35.3%), sulfonamides (33.3%), third-generation cephalosporins (30.7%), fourth-generation cephalosporins (22.5%), fluoroquinolones (21.8%), phenicols (16.8%), and carbapenems (16.2%). The distribution of antibiotic resistance was 45.3% from human sources, 19.3% from animal sources, and 13.8% from environmental sources. Conclusion: The results indicate that resistant bacteria can come from any of the three biotopes, with human origin being the most frequent. The high prevalence of resistance to the antibiotics tested in isolated bacteria raises interest in investigating the genetic factors responsible. Competing Interests: The authors report no conflicts of interest in this work. (© 2024 Dabiré et al.)
Databáze:	MEDLINE
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