The interleukin-6 signal transducer receptor subunit is required for optimal in vitro bovine embryo development.

Autor:	Speckhart SL; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA.; Institute for Reproductive and Developmental Sciences, University of Kansas Medical Center, Kansas City, KS, USA.; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA., Pollock AB; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA., Alward KJ; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA.; Department of Agricultural Sciences, West Texas A&M University, Canyon, TX, USA., Farrell K; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA., Oliver MA; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA., Lee K; Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA., Biase FH; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA., Ealy AD; School of Animal Sciences, Virginia Tech, Blacksburg, Virginia, USA.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2025 Jan 05. Date of Electronic Publication: 2025 Jan 05.
DOI:	10.1093/biolre/ioaf006
Abstrakt:	This work explored whether bovine embryo development relies on signaling from the interleukin-6 (IL6) cytokine family. This was accomplished by interrupting IL6 signal transducer (IL6ST), the common beta-subunit receptor used by the IL6 family. One series of studies cultured in vitro-produced (IVP) embryos with SC144, a pharmacological IL6ST inhibitor. Providing the inhibitor at a concentration that partially diminished IL6ST signaling reduced development to the 16-cell and blastocyst stages and reduced inner cell mass (ICM) cell numbers. Inhibitor concentrations that completely blocked IL6ST signaling prevented blastocyst development. Another series of studies used CRISPR-Cas9 to disrupt IL6ST. Two electroporation approaches were used to introduce guide RNAs and Cas9 protein into one-cell IVP embryos. Editing efficiency was ≥82%. Targeting IL6ST did not affect cleavage but reduced development to the 16-cell and blastocyst stages. A reduction in ICM cell numbers was detected, and a disorganization of the ICM was observed in approximately one-half of the IL6ST-targeted blastocysts. These observations indicate that embryo-derived IL6 family members that signal through IL6ST are needed to support normal in vitro bovine embryo development. These signals are needed by the 16-cell stage and for ICM cell development at the blastocyst stage. There also is evidence that these signals support the overall cellular organization of the blastocyst. (© The Author(s) 2025. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu