Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25.

Autor: Dadayya M; Department of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu University, Jnanasahyadri, Shivamogga Dist, Shankaraghatta, 577451, Karnataka, India., Thippeswamy MG; Department of P.G. Studies and Research in Biochemistry, Kuvempu University, Shivamogga Dist, Jnanasahyadri, Shankaraghatta, 577451, Karnataka, India., Shivaiah N; Department of Studies and Research in Biochemistry, Tumkur University, Tumakuru, 572103, Karnataka, India., Siddaraju TR; Department of Studies and Research in Biotechnology, Tumkur University, Tumakuru, 572103, Karnataka, India., Jayaramaiah P; Department of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu University, Jnanasahyadri, Shivamogga Dist, Shankaraghatta, 577451, Karnataka, India., Veeranna SH; Department of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu University, Jnanasahyadri, Shivamogga Dist, Shankaraghatta, 577451, Karnataka, India., Basaiah T; Department of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu University, Jnanasahyadri, Shivamogga Dist, Shankaraghatta, 577451, Karnataka, India. thippeswamyb272@yahoo.in., Mathad SN; Department of Physics, KLE Institute of Technology, Hubbali, 580 027, Karnataka, India., Hemagiri Gowda R; Department of Life Sciences, Bangalore University, Jnana Bharathi Campus, Bangalore, 560056, Karnataka, India., Naik S; Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh, 11433, Saudi Arabia. snaik@ksu.edu.sa., Kheraif AAA; Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh, 11433, Saudi Arabia., Vellappally S; Dental Health Department, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh, 11433, Saudi Arabia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2025 Jan 03; Vol. 15 (1), pp. 606. Date of Electronic Publication: 2025 Jan 03.
DOI: 10.1038/s41598-024-76475-x
Abstrakt: In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-AgNPs were characterized through various bioanalytical methods. The synthesis was confirmed by Bio- spectrophotometry, which showed an absorption peak at 404 nm. FTIR analysis verified the reduction and capping of Con-AgNPs, displaying peaks corresponding to various functional groups. SEM-EDAX and HR-TEM examinations revealed that the Con-AgNPs were spherical, and EDAX analysis confirmed the presence of silver atoms at 3 keV. XRD studies revealed the crystalline structure of Con-AgNPs. DLS and Zeta potential tests determined the size and stability of the synthesized Con-AgNPs, which were 65.81 nm. The Con-AgNPs demonstrated strong antibacterial activity against P. aeruginosa (14.06 ± 0.11 mm, 10 mg/mL) and effective antifungal activity against A. flavus (13.03 ± 0.05 mm, 10 mg/mL). Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200 µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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