Flavuside B exhibits antioxidant and anti-inflammatory properties in Staphylococcus aureus infected skin wound and affect the expression of genes controlling bacterial quorum sensing.

Autor:	Chingizova EA; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Yurchenko EA; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Starnovskaya SS; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Chingizov AR; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Kuzmich AS; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Pislyagin EA; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Vasilchenko AS; Laboratory of Antimicrobial Resistance, Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, Tyumen, Russia., Poshvina DV; Laboratory of Antimicrobial Resistance, Institute of Environmental and Agricultural Biology (X-BIO), Tyumen State University, Tyumen, Russia., Shilovsky GA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.; Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.; Institute for Information Transmission Problems, Russian Academy of Sciences (Kharkevich Institute), 127051 Moscow, Russia., Dibrova DV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.; School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119991 Moscow, Russia., Aminin DL; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia., Yurchenko AN; G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS; 690022 Vladivostok, Russia.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2025 Jan 02. Date of Electronic Publication: 2025 Jan 02.
DOI:	10.1093/jambio/lxae318
Abstrakt:	Aims: The aim of this study was to evaluate the antioxidant and anti-inflammatory effects of marine fungal cerebroside flavuside B (FlaB) on Staphylococcus aureus-infected keratinocytes in in vitro skin wounds and to identify FlaB targets in bacterial and human cells. Methods and Results: A combination of ELISA, plate spectrofluorimetry, and flow cytometry with fluorescence dye staining, scratch assay, and real-time cell imaging techniques was used to investigate the effects of FlaB on S. aureus-infected HaCaT keratinocytes. FlaB decreased ROS levels, NO levels, and TNF-α and IL-18 release in S. aureus-infected HaCaT cells. FlaB reversed the inhibition of HaCaT cell proliferation caused by S. aureus infection. FlaB significantly increased keratinocyte migration and wound healing in an in vitro S. aureus-infected wound skin model. Using real-time qPCR, we found that FlaB caused a 1.7-fold reduction in agrA expression, which controls quorum sensing system in S. aureus. Bioinformatics analysis and molecular docking, together with experimental data, suggest that FlaB targets the pro/antioxidant defense system in human cells. Conclusions: Thus, FlaB can play a dual role as an antibacterial and pro/antioxidant machinery modulator, providing an observable positive effect in S. aureus-infected in vitro skin wounds. Staphylococcal sortase A enzyme and Arg systems are the targets of FlaB in bacterial cells. Nrf2/Bach1 dependent pro/antioxidant defense system is a target of FlaB in human cells. Some suggestions have also been made regarding the biological role of this marine fungal metabolite and its therapeutic possibilities. (© The Author(s) 2025. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu