Effects of prolonged oxytetracycline supplementation on freshwater stinging catfish ( Heteropneustes fossilis ): a multi-biomarker approach.

Autor:	Choudhary P; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India., Naik SP; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India., Sahoo SR; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India., Das R; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India., Sahoo SN; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India., Panda SK; Fish Processing Division, Indian Council of Agriculture Research (ICAR)-Central Institute of Fisheries Technology, Cochin, Kerala, India., Abraham TJ; Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India., Patil PK; Indian Council of Agricultural Research (ICAR)-Central Institute of Brackishwater Aquaculture (CIBA), Aquatic Animal Health and Environment Division, Chennai, Tamil Nadu, India., Swain P; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India., Mishra SS; Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2024 Dec 19; Vol. 15, pp. 1478114. Date of Electronic Publication: 2024 Dec 19 (Print Publication: 2024).
DOI:	10.3389/fimmu.2024.1478114
Abstrakt:	Background: Aquaculture systems that sporadically depend on antibiotics can contribute to the development of adverse effects on the fish, microbial flora and the environment. This study sought to investigate the impacts of extended oxytetracycline supplementation on the freshwater stinging catfish Heteropneustes fossilis through a multi-biomarker approach. Methods: A total of 300 H. fossilis (20 ± 0.5 g) were placed in fibreglass-reinforced plastic tanks. The experimental fish were administered oxytetracycline hydrochloride (OTC) at varying doses, viz., 80 mg/kg fish biomass/day (1x), 240 mg (3x), 400 mg (5x), and 800 mg (10x) for 30 consecutive days. The study also included a control group that did not receive OTC. Results: OTC was effective against Aeromonas hydrophila , Pseudomonas putida , and Plesiomonas shigelloides , with minimum inhibitory concentrations ranging between 0.5 and 8.0 µg/mL. The OTC supplementation retarded the growth of fish. The respiratory burst activity, myeloperoxidase, and lysozyme increased significantly in the 1x group until day 20. This group showed an increase in serum albumin, whereas other OTC groups exhibited elevated levels of liver functional enzymes, including alanine transaminase, aspartate aminotransferase, and alkaline phosphatase. In addition, OTC groups exhibited increased levels of antioxidant enzymes. The magnitude of the increase was dose- and time-dependent. The liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) study signified a dose-dependent increase in OTC residues in the muscle. After a 10-day discontinuation of OTC, the tissue level retention of residues was minimal in the muscle, specifically in the 1x group compared to other groups. Significant histological alterations were noted in the liver tissues of the 5x and 10x groups, possibly due to oxidative stress and residue accretion. Conclusion: The therapeutic dose of 80 mg/kg biomass/day was safe and tolerated well by H. fossilis , and may be used for sustainable catfish farming practices. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Choudhary, Naik, Sahoo, Das, Sahoo, Panda, Abraham, Patil, Swain and Mishra.)
Databáze:	MEDLINE
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