RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: data from Campania, Italy.

Autor:	Pisapia P; Department of Public Health, University of Naples Federico II, Naples, Italy., Iaccarino A; Department of Public Health, University of Naples Federico II, Naples, Italy., De Luca C; Department of Public Health, University of Naples Federico II, Naples, Italy., Pepe F; Department of Public Health, University of Naples Federico II, Naples, Italy., Russo G; Department of Public Health, University of Naples Federico II, Naples, Italy., Nacchio M; Department of Public Health, University of Naples Federico II, Naples, Italy., Ambrosio F; Oncology Unit, A.O.R.N. Cardarelli, Hospital Antonio Cardarelli, Naples, Italy., Bianco R; Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy., Campione S; Pathology Unit, A.O.R.N. Cardarelli, Hospital Antonio Cardarelli, Naples, Italy., Caputo A; Department of Pathology, University Hospital of Salerno, Salerno, Italy., Carotenuto P; Department of Translational Medical Science, Medical Genetics, University of Naples Federico II, Naples, Italy.; TIGEM, Telethon Institute of Genetics and Medicine, Pozzuoli, Naples, Italy., D'Antonio A; Pathology Unit, Ospedale del Mare, Naples, Italy., D'Armiento M; Department of Public Health, University of Naples Federico II, Naples, Italy., Damiano V; Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy., Daniele B; Oncology Unit, Ospedale del Mare, Naples, Italy., De Chiara G; Division of Pathology, 'S.G. Moscati' Hospital, Avellino, Italy., De Felice M; Medical Oncology Unit, Ospedale Ave Gratia Plena, Piedimonte Matese, Caserta, Italy., Della Gravara L; Department of Precision Medicine, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy., Fabozzi T; Oncology Unit, Ospedale del Mare, Naples, Italy., Feliciano S; Medical Oncology Unit, Ospedale Ave Gratia Plena, San Felice a Cancello, ASL Caserta, Caserta, Italy., Gridelli C; Division of Medical Oncology, 'S.G. Moscati' Hospital, Avellino, Italy., Guadagno E; Pathology Unit, A.O.R.N. Cardarelli, Hospital Antonio Cardarelli, Naples, Italy., Ilardi G; Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy., Leopardo D; Department of Oncology, A.O.R.N. Sant'Anna e San Sebastiano, Caserta, Italy., Libroia A; Oncology Unit, 'Andrea Tortora' Hospital, ASL Salerno, Pagani, Italy., Maione P; Division of Medical Oncology, 'S.G. Moscati' Hospital, Avellino, Italy., Morgillo F; Department of Precision Medicine, Università degli Studi della Campania 'Luigi Vanvitelli', Naples, Italy., Orefice J; Oncology Unit, Ospedale del Mare, Naples, Italy., Panico L; Department of Pathology, A.O.R.N. dei Colli Monaldi, Naples, Italy., Rocco D; Department of Pulmonary Oncology, A.O.R.N. dei Colli Monaldi, Naples, Italy., Servetto A; Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy., Varricchio S; Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy., Zeppa P; Department of Pathology, University Hospital of Salerno, Salerno, Italy., Vigliar E; Department of Public Health, University of Naples Federico II, Naples, Italy., Bellevicine C; Department of Public Health, University of Naples Federico II, Naples, Italy., Troncone G; Department of Public Health, University of Naples Federico II, Naples, Italy., Malapelle U; Department of Public Health, University of Naples Federico II, Naples, Italy.
Jazyk:	angličtina
Zdroj:	Pathologica [Pathologica] 2024 Oct; Vol. 116 (5), pp. 310-319.
DOI:	10.32074/1591-951X-1015
Abstrakt:	Objective: ALK, ROS1, NTRK, and RET gene fusions and MET exon 14 skipping alterations represent fundamental predictive biomarkers for advanced non-small cell lung cancer (NSCLC) patients to ensure the best treatment choice. In this scenario, RNA-based NGS approach has emerged as an extremely useful tool for detecting these alterations. In this study, we report our NGS molecular records on ALK, ROS1, NTRK, and RET gene fusions and MET exon 14 skipping alterations detected by using a narrow RNA-based NGS panel, namely SiRe fusion. Methods: We retrospectively reviewed data on 201 advanced stage NSCLC patients who were referred to our laboratory for RNA-based molecular evaluation of ALK, ROS1, RET, NTRK gene rearrangements as well as MET exon 14 skipping. Results: Overall, 23 (11.4%) positive cases were retrieved. Regarding molecular assessment, 11 (5.5%), 2 (1.0%), 9 (4.5%), and 1 (0.5%) out of 201 harbored an ALK, ROS1, RET gene rearrangement, or MET exon 14 skipping, respectively. Conclusions: In this study, we provide real-world experience on RNA-based NGS analysis in patients with advanced stage NSCLC. (Copyright © 2024 Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.)
Databáze:	MEDLINE
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